FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_20-CRC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_20-CRC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4692533
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG49650.065.731865
GGTATAG103500.065.328855
GTATAGG110550.064.485036
TATACGG60500.064.00387
GTATAGC51550.063.444866
TATAGCG48400.063.093187
CGCTATA41900.062.8629074
CGTATAC33550.062.7649965
TTATAGC62950.062.1795926
GCTATAG62950.061.901765
GATATAG78800.061.5248455
CGGTATA62650.061.3606764
GTATACG49600.061.355166
CTATAGC38550.061.2481426
CTATACG40400.061.2141156
GCTATAA65100.061.200685
TATAGCA61000.061.185447
GCTATAC43250.061.062475
TATAGCC34850.061.0259177
CCTATAC31200.060.8777735
Produced by FastQC (version 0.11.5)