FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_2-WRKY60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_2-WRKY60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044685
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG65110.21384806638453566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG31800.065.887485
GGTATAG57000.065.7230765
GTATAGG63450.064.830446
GTATAGC31650.064.099936
TATAGCG31250.063.7989967
CGTATAC22350.063.697175
CTATACC21950.063.4237376
TATACGG33000.063.3835037
CCTATAC18150.063.0194175
CGGTATA37700.062.905934
GGTATAA60500.062.6147045
CTATAGC24150.062.5705346
CGCTATA24750.062.1842964
CGTATAA43750.061.32265
TATAGGC39350.061.243997
GGTATAC30900.061.2409675
TTATAGC40600.061.0834356
GCTATAC26400.061.0801735
AGTATAG49900.061.0549475
TATACCG25850.061.0246057
Produced by FastQC (version 0.11.5)