FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_19-SAP11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_19-SAP11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350505
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG46310.10644741242683321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG45850.065.310345
GGTATAG96350.065.135575
GTATAGG98250.064.8372966
GTATAGC47000.064.010046
TATAGCG52550.063.306797
CGTATAC33350.063.2512325
TATACGG58700.063.2295077
CTATAGC38950.062.7794046
GCTATAG55700.062.3022885
CCTATAC27600.062.232885
GTATACG46550.061.998586
CGGTATA57850.061.800934
CGTATAA57350.061.790765
TTATAGC61100.061.7771346
GGTATAA84700.061.415085
CGCTATA38100.061.150044
GCTATAC40500.060.981375
GGGTATA83800.060.9475674
GTATAGT107650.060.8005186
TATAGCC35750.060.765647
Produced by FastQC (version 0.11.5)