FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_18-MYB34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_18-MYB34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4388589
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG50000.11393183549427846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG47600.066.144795
GGTATAG92600.066.1130455
GTATAGG100850.065.214186
CCTATAC30850.064.4099965
CGTATAC32200.064.208385
TATACGG57750.064.149637
TATAGCG49900.063.865757
GTATAGC48750.063.7233246
TTATAGC60000.062.7949266
GTATACG46550.062.7518966
CTATACC30050.062.7486466
CGGTATA58500.062.5512354
GATATAG70800.062.505375
CGCTATA37500.062.4101144
GCTATAG57350.062.0975465
GGGTATA85100.062.0325664
CTATAGC37400.061.64166
GGTATAC50250.061.4034275
TATAAGG82950.061.277277
GGTATAA89200.061.103025
Produced by FastQC (version 0.11.5)