FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_17-TCP23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_17-TCP23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055402
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG31050.066.026685
GTATAGG69850.065.3623356
GGTATAG65500.064.735835
TATAGCG34100.064.4300847
CGCTATA29850.063.9928974
GTATAGC30850.063.959846
CGTATAC23250.063.8008275
CTATAGC26200.063.2936976
TATACGG37100.062.897787
TATAGGC40450.062.705117
CGGTATA41550.061.466044
GATATAG50900.061.3098835
GTATACG31750.061.265296
GGGTATA56350.061.2162064
TTATAGC42700.061.1215556
CCTATAC20650.060.9910165
TATAAGG57800.060.830547
TATAGCC24600.060.5839967
TATAGGT76850.060.5468037
GCTATAC29300.060.5373955
Produced by FastQC (version 0.11.5)