FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_16-CRC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_16-CRC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218290
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG23210.10463014303810593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC15450.065.657975
CGTATAG24100.065.460215
GTATAGG48900.064.880836
TATACGG29700.064.77757
TATAGCG23200.064.682567
GTATAGC25200.064.268536
GGTATAG44950.064.045425
CCTATAC14550.063.228185
CTATACC15800.062.875176
CTATAGC19800.062.7163966
GTATACG25250.062.4788556
GCTATAG28600.062.2543455
TTATAGC30000.062.0309336
TATAGCC18200.061.6952257
TTATACC20000.061.564536
GATATAG36150.061.5413135
GGGTATA39950.061.4664734
CGTATAA31500.061.298025
CGGTATA28050.061.1056184
TATACCG18750.060.6317377
Produced by FastQC (version 0.11.5)