FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_15-GAMMAH2AX.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_15-GAMMAH2AX.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979338
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG52130.17497175547051055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG32100.067.247475
CGTATAC26300.066.646565
GGTATAG47950.065.9592365
CTATACC23000.065.713066
GTATAGC32550.065.350426
CCTATAC22450.065.14125
TATACCG21050.064.653697
GTATAGG50500.064.4298866
GGTATAA55400.063.9728165
GCTATAG34200.063.732035
TATAGCG30500.063.663277
CGTATAA50050.063.4014175
CCTATAA32250.063.137755
TATAGGC30600.062.9978837
CGGTATA40950.062.881044
TATACGG32700.062.8038337
AGTATAG39750.062.4909365
TTATAGC36750.062.4514166
GTATACG33000.062.1268736
CTATAGC24900.062.1038786
Produced by FastQC (version 0.11.5)