FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_14-LBD4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_14-LBD4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377398
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG69730.29330385572798495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG24400.066.246785
CGTATAC16450.065.295785
GGTATAG40250.064.933275
CCTATAC16050.064.30725
GTATAGG42600.064.225836
CTATACC17450.064.160416
GTATAGC23200.064.093446
TATACGG26550.063.778637
CTATAGG24650.063.445856
GCTATAG28950.063.327935
CTATAGC19250.063.2500846
GTATACG23350.062.632826
CCTATAG17550.062.1999555
TATAGCG23750.062.16467
GGTATAC23750.061.8725855
GGGTATA37650.061.611414
TATACTG37200.061.41367
GATATAG32950.061.3740845
CGCTATA23050.061.322954
CCGCGTA1600.061.226792
Produced by FastQC (version 0.11.5)