FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_13-HB23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_13-HB23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868361
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG34970.18716939606425095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG41100.066.484745
TATACGG26150.065.4259347
GTATAGC20600.065.3892446
GTATAGG44450.065.173546
TATAGCG19800.064.673847
TATAGCC13050.064.34487
CGTATAG20150.064.245025
TATACCG17150.063.650717
CGCTATA15450.062.7283824
CGTATAC13350.062.6367765
CTATAGG22600.062.38926
GTATACG23050.062.3854986
TTATAGC26700.062.243666
CCTATAC11300.062.2343865
CCGCGTA1350.062.1999782
GATATAG32800.062.188135
TATAAGG33400.062.013757
GGGTATA39100.061.6531454
GCTATAG25850.061.4480445
CGTATAA25750.061.4149325
Produced by FastQC (version 0.11.5)