FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_12-WRKY60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_12-WRKY60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3426841
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG54010.15760871309757296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG79000.065.0133066
GGTATAG75100.064.98965
CCTATAC22400.064.976295
CGTATAG36650.064.437615
TATACGG48400.064.335097
CTATAGG40500.063.839976
GTATAGC38100.063.8209236
TATAGCG38150.063.003617
CGGTATA43500.062.977024
GGGTATA65900.062.9133034
GTATACG39550.062.896496
CTATACC25150.062.4613846
GCTATAG47150.062.4056475
CGCTATA30050.062.290084
TTATAGC48650.061.990866
GGTATAA65150.061.919085
ATAGCGC6950.061.918958
TATAGGC46150.061.7100567
TATAGTG91650.061.5751047
GATATAG55800.061.5725145
Produced by FastQC (version 0.11.5)