FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_11-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_11-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4711176
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG101540.21553005024647776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG49700.065.6711355
GGTATAG97150.065.463665
GTATAGC48350.064.537636
CTATACC33650.064.454216
CGTATAC33750.064.367565
GTATAGG100800.064.098966
TATAGCG49700.063.0661437
TATACGG55600.062.9801867
CCTATAC31950.062.9573175
CTATAGC37400.062.761826
GGGTATA91600.062.5558174
GGTATAA89350.062.4866945
CGTATAA68350.062.287565
GCTATAG59500.062.0863655
CGCTATA38500.062.059644
GATATAG72700.061.9770245
CGGTATA60650.061.7743154
GTATACG48300.061.707386
TATAGCC37550.061.57957
GGTATAC52950.061.574265
Produced by FastQC (version 0.11.5)