FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_10-GAMMAH2AX.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_10-GAMMAH2AX.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4206459
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG84560.2010241868516964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG45450.066.112735
GTATAGG83150.065.543726
GGTATAG79150.065.232095
TATAGCG43750.064.442517
GTATAGC44050.064.0845646
CGGTATA55300.063.002834
GGTATAC42900.062.7854925
CGCTATA35700.062.7101174
GGGTATA71800.062.7017944
CGTATAC32150.062.671115
TATACGG51750.062.5916187
ATAGCCG12850.062.3372848
GTATACG45700.061.7707946
GCTATAG55150.061.7153475
CTATAGC32250.061.6090476
TATACCG31250.061.452267
TATAGCC34850.061.4276927
GGTATAA86750.061.4124265
GCTATAC35550.061.3019525
CTATAGT60950.061.122596
Produced by FastQC (version 0.11.5)