FastQCFastQC Report
Fri 15 Nov 2019
HJ5WJBGXC_n01_1-EV-RFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5WJBGXC_n01_1-EV-RFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3127973
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG56280.17992482671685464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC34600.064.906716
CGTATAG33350.064.822135
GTATAGG62700.064.717416
GGTATAG57800.064.696465
TATACGG37050.064.390217
TATAGCG32250.064.103467
CCTATAC22150.063.9605145
CGTATAC25700.063.4283525
CGTATAA43250.063.3295365
TATAGCC26950.062.821797
GCTATAC26350.062.7929885
GGTATAA59900.062.7786945
CGGTATA40500.062.620084
GGGTATA54000.062.4473344
TTATAGC41250.062.414366
AGTATAG52450.062.2252735
CTATAGC24350.061.773216
GTATACG35050.061.5783166
CGCTATA28250.061.5415734
GCTATAA47150.061.281445
Produced by FastQC (version 0.11.5)