FastQCFastQC Report
Mon 18 Apr 2016
HJ5TTBCXX_l02n02.332000000522e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TTBCXX_l02n02.332000000522e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197548229
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4349340.2201659828598109No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3314160.16776460192918255No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATC2931490.14839363606747394No Hit
CACACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2011110.1018034942748082No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2009020.10169769732534531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG1212800.030.60621570
TCATTAA6986950.029.08029667
ATCATTA7110350.028.08182366
CATTAAA7599800.027.64164268
TATCATT7376900.027.35868565
TCCAATG1968800.027.27374613
TGAACGT1417100.026.94485918
ATGCACG2157700.026.5257117
TTGAACG1382500.026.4417917
GGTATCC2065300.026.337239
ATCGGTT1333300.025.98533412
AATGCAC2138400.025.87739816
GTATCAT7829050.025.77698564
GGTTGAA1329800.025.41973515
CGGTTGA1348550.025.28359214
ATTAAAA8609950.025.08957969
TGCACGT2477800.025.04298218
CTATCGG1351500.025.0392610
CGTATCA8230450.024.91906763
GAACGTA1610250.024.60305219
Produced by FastQC (version 0.11.2)