FastQCFastQC Report
Mon 18 Apr 2016
HJ5TTBCXX_l02n01.331000000522e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TTBCXX_l02n01.331000000522e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197548229
Sequences flagged as poor quality0
Sequence length26
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTCCGAGC2102150.1064119891451925No Hit
GCTGTCTCTTATACACATCTCCGAGC2065100.10453649776835004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT18875900.025.03733320
GTTTTTT24008400.024.89916220
AATTTTT4468150.024.72225619
CTTTTTT14099050.024.70622420
AGTTTTT5381650.024.65721119
TATTTTT4870950.024.62536419
GATTTTT5823500.024.52755719
GGTTTTT8371500.024.40995219
TGTTTTT5940850.024.33372519
CGTTTTT5043650.024.2530819
CATTTTT4367600.024.00685919
ACTTTTT3722600.022.03929519
TCTTTTT3757200.021.87669419
CCTTTTT3216000.021.48789619
GCTTTTT5683700.021.21303419
CTCATGG1022300.021.0588848
TCCGAGC2924100.020.8584220
AGCTAGG722050.020.8526348
AGCTAGC396550.020.3969698
CTCATTG537800.020.2824578
Produced by FastQC (version 0.11.2)