FastQCFastQC Report
Mon 18 Apr 2016
HJ5TTBCXX_l01n02.332000000522f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TTBCXX_l01n02.332000000522f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197093378
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT5509870.27955632279030707No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4233680.21480579626576798No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATC3645970.18498693548192166No Hit
CACACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2658030.13486145638033561No Hit
CGTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2640290.13396137540450495No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2538690.12880645842905997No Hit
ACCACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2504110.12705196011202366No Hit
AGTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2431000.12334255085931908No Hit
GTTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2107700.10693915855458118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG1532350.034.7348870
TCATTAA8951300.027.67257567
CATTAAA9922050.027.4150668
GGTATCC2191850.027.3192069
TCCAATG2284100.026.7186913
ATCGGTT1627350.026.65562212
ATCATTA9093250.026.54047666
CTATCGG1574050.026.45450210
CCTATCG1604750.025.846269
TGAACGT1808250.025.49050518
TATCATT9191000.025.46025765
ATTAAAA11099850.025.02190469
TATCCAA2411400.025.00671411
CGGTTGA1759800.024.99874914
TTGAACG1779100.024.9356617
TCAACGC795650.024.871143
CGGTATC1105300.024.8226288
GGTTGAA1743800.024.71137615
ATGCACG2516050.024.27166417
AATGCAC2490950.024.26403416
Produced by FastQC (version 0.11.2)