FastQCFastQC Report
Mon 18 Apr 2016
HJ5TTBCXX_l01n01.331000000522f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TTBCXX_l01n01.331000000522f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197093378
Sequences flagged as poor quality0
Sequence length26
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTTT5036700.024.02559919
TATTTTT5477700.023.9975519
AGTTTTT6141550.023.99003819
GATTTTT6588700.023.78818919
TGTTTTT6740000.023.7302419
GGTTTTT9530500.023.68113519
CGTTTTT5716550.023.58877219
ATTTTTT22184250.023.34240220
CATTTTT4927250.023.26026719
GTTTTTT28452050.023.24687220
CTTTTTT16898150.023.08205220
ACTTTTT4268200.021.53312719
TCTTTTT4300800.021.370519
CTCATGG1218600.021.05848
CCTTTTT3689050.020.9784419
GAGCTAG1681850.020.7917737
AGCTAGG773400.020.7264258
GCTTTTT6553350.020.6920519
GCTCATG2803100.020.6373737
ACGTAGG1691200.020.5961387
Produced by FastQC (version 0.11.2)