FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n02_mesp_mekmut_10hpf_3_may.3520000005991f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n02_mesp_mekmut_10hpf_3_may.3520000005991f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26743
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGTC406.0461246E-428.05640442
CCTGTCT1102.641811E-617.92694743
TTATACA600.00937254217.5085645
TGTCTCT2400.015.75770545
CTTATAC900.004286938314.60714244
CTGTCTC3001.4551915E-1012.41607244
Produced by FastQC (version 0.11.2)