FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n02_mesp_lacz_10hpf_2_june.35200000059939.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n02_mesp_lacz_10hpf_2_june.35200000059939.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58968874
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC83050.017.3624518
CTGCCGA69450.017.18828224
CCGACGA70000.017.17177427
CTCGGTG65550.016.80384842
TCTCGGT73850.016.09707641
TCGGTGG67300.015.75708243
CGCTGCC79300.015.5692822
GCTGCCG75500.015.49159623
TGACGCT92700.015.20608119
GACGAGT72750.015.15564229
TCTGACG98600.014.85378517
GACGCTG85250.014.32794520
TGCCGAC85750.013.89621725
GCCGACG79050.013.67567626
ATCTCGG84950.013.41514340
ACGAGTG87750.013.25785930
ACGCTGC95850.013.17910221
CGAGTGT90450.012.94573531
ATCTGAC120050.012.59475516
GTAGATC107700.012.53214236
Produced by FastQC (version 0.11.2)