FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n02_gfp_18hpf_20_05_15.352000000598f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n02_gfp_18hpf_20_05_15.352000000598f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17898000
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG27200.016.83395443
CTCGGTG29650.015.69248342
TCTCGGT32400.015.39573341
GTAGATC37200.014.79774836
AGATCTC37500.014.72815138
CTGACGC40300.014.38021118
CCGACGA36100.014.20678527
GACGAGT32700.014.12876829
CTGCCGA36850.013.96973824
GCTGCCG37850.013.67829123
ATCTCGG36300.013.12987340
TGACGCT44150.013.10356619
CGCTGCC39650.013.0237422
CGGTGGT34600.012.93144744
GACGCTG38600.012.80846420
TCTGACG47050.012.79830317
GATCTCG42250.012.49740239
GCCGACG38300.012.33190126
CGAGTGT39500.012.31242831
ACGAGTG39400.012.10487130
Produced by FastQC (version 0.11.2)