FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n02_gfp_15hpf_23_04_15.3520000005996f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n02_gfp_15hpf_23_04_15.3520000005996f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28349385
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG29450.012.42491743
TCTCGGT38100.010.81006541
CTCGGTG33650.010.44371642
CTGACGC49250.010.38794318
AGTCTGA38850.010.23531233
ATCTCGG36350.010.16515540
GTATAAG118400.010.0201041
AACCGCG40700.09.8998517
GCGGTAG33850.09.87983511
CGGTGGT36700.09.80275744
CCGACGA42600.09.5950127
ACCGCGG35500.09.4198898
CTGCCGA43350.09.39097524
CCGCGGT34750.09.2798099
GTACTGC45850.09.160376
CGGTAGC37550.09.12408312
CTAGTCA42900.08.9645539
TGACGCT56850.08.85978619
GACGCTG48550.08.82480520
GTACTAC51350.08.71552853
Produced by FastQC (version 0.11.2)