FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n02_gfp_10hpf_2_june.35200000059953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n02_gfp_10hpf_2_june.35200000059953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGGCNATANTGTTTANGTNGGTTTCTTGGTTTGNTCTATCTTTACCGC11.25No Hit
ATATTAGCTTTNATATGAAATAGTGTAAAATAGAATATTTTTAAACAAACT11.25No Hit
ATACAACACCANAGCAGTTTGATTCGTTGTTGTTTTATCAAACTGTAATTT11.25No Hit
NGGTNTNNCGGCNNNANNNNNTNCGNNNNCCGGCAATGCGGGCNACGCAGG11.25No Hit
GAATTGTCACAACAAGTNATTAGCGACGGATATTTCGCCTTTGAAACTTAA11.25No Hit
GNCNCNGNGNANTNANNNNNNNGTNCNNNNCANGGNTNNGTNTNANNTTAC11.25No Hit
AGTTNNNNNNNCCTGCNTNNNNNAGAGCTAATNGNCGTCTTCATNNCCAGA11.25No Hit
ATAGCCTTGAATGGCAGATTTAATCCCAGCATCACCCATGCCTACAGTCTT11.25No Hit
ACTCTATTCTAACCGTCGACGTGAAGAGATCAATTAAGCTTAGAGTAGTTG11.25No Hit
AATCGAGTCATATATTCGTAAGACACGAGGACGGCATAACACGAAGCCAGC11.25No Hit
TTCGTAGGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCC11.25No Hit
GGTTAACGCCGATCTCTGACTGCATGACCATCCCTTCTTTGCCGGTTTCGG11.25No Hit
GTTAATANNNNANNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNN11.25No Hit
NTANNNNNNNCNNNNCNNGGCNCNNNNNNANNNNTGTTNNAGNTNTTCNAA11.25No Hit
NNNNNNNNNGGNCNNNNNNNNTACNNNNNNNNNNNNTNNGGGCGNNGNANG11.25No Hit
AGNAACGAGAGGAGCCAAGCGGAAGTGCCTGTCAACCGCGCGGGTAATGGG11.25No Hit
NNNCNNNNNNNNNNNNNNNNNNNNANNNNNNCNNNNNNNNNNATNNNNNNG11.25No Hit
CCCTGNNTTGTATGGCAACTTGCCGCCGCGTGAAATTGCAATGAAGTATGT11.25No Hit
NNNTNNNNNNNNNNNNNNNNNNNNCNNNNNNANNNNNNNNNNTTNNNNNNC11.25No Hit
CTNGTGTGTCACCCGCACCTCCCCAAACGACTCGCCGCAGACCAGACCGTT11.25No Hit
NNNTNNNNNNNNNNNNNNNNNNNNANNNNNNANNNNNNNNNNGCNNNNNNT11.25No Hit
TAATGAGAGATCCTTTTCAGTTAAGTTAAATAGACAAGAACAGTTAAATCT11.25No Hit
NACCTTNNATTNTNTGCCTGTCTGGTTCGNNTNGAGACAACATAACTTCCG11.25No Hit
ATCTGTAGTAGGTCCTGTCTTCACTGAAGAAGTTGCTCCTTTTTNTCGAGA11.25No Hit
ANGNANGNANTNGNTNNNNNNNGANTNNNNTCNTGNCNNGCNTNTNNCTTA11.25No Hit
NGAACCNNCCGNTNAGCCCGCCCGCTGCGNNTNATCCGGTAACTATCGGCT11.25No Hit
TTTTCATCCCGATTTTGCGGCTCATTCGGCTTCTAAACCGCTTGTTTGGAA11.25No Hit
ATTTTAANTTCNTCTATTNATNTAGTATATTTATTANTTAGGTGGGAAAGA11.25No Hit
NTCTAANNTAANGNAGTTAGATTGAATTCNNTNTTATCTATCCACGTTCTC11.25No Hit
TTNTTACTTCTGCACCTAGCTCAGAAACTTGAATATACTAACAAACTTGCA11.25No Hit
GANTTGGGTGGCTCTGAAAAGAGCCGTTTGCAGAGCTGTCCCTTATACACA11.25No Hit
CAACCGATTAGCGGCGATTCATGAGAATCCAAATACTTTGCGTGACTATTT11.25No Hit
GCCATCGACTAACTATTCTGTGAAAAACTGGCGCATTGGATGAGGAGAGGT11.25No Hit
CNANCNANTNANTNANNNNNNNAGNANNNNGCNCTNANNAANTNTNNTACG11.25No Hit
GTTGATGGAACTGACCAAACGTCGTGAGGCCAGTTTTCTGGTCGTGTTCAA11.25No Hit
ATGTTTTCATGCCTCAAAATCGTGGAGGCTTTTTTAAGCTTCGTTCTTATT11.25No Hit
TACTCGGTCTTGCAAATCACCGGAAGGCGTTTCCGGAATGAATGGGAAGCC11.25No Hit
CGGTATTTCTANGGCTCTGATTACGGTGTCGCCGAAATCTCGTTCCCAAGA11.25No Hit
GTATCTGTTGTGCTTGATGTTTGTGGCCCTACCGTGCGAGAAGTAGACTCA11.25No Hit
CTNGTGTGGCACCGGCACCTCGCCAAAGGACTCGCCGCAGACCAGACCGTT11.25No Hit
NNNTNNNNNNNNNNNNNNNNNNNNCNNNNNNCNNNNNNNNNNTTNNNNNNA11.25No Hit
AGTGNNNNNNNCACACNANNNNNAGAGAAGTCNTNAAAATGACANNGGCGA11.25No Hit
GGCACGACAGGAAGATACAAATGCAGGGCTGACCGAACCCTGAGCGAGAAG11.25No Hit
GAACAACATGTTTCTGTAAACTGTTTACTATTGTCGTTTGCGGTACAGCGA11.25No Hit
TCTCTGTAACGTCTCCGTAGGCTTTGTGATGGACTTGGAACTGTTTTCGAC11.25No Hit
GCACCGGGGGCGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGT11.25No Hit
CCTTTACATAGCCGATGCATTCACATATAATGTTCTTTGTGCAACCGAAGT11.25No Hit
NNNCNNNNNNNNNNNNNNNNNNNNANNNNNNANNNNNNNNNNGANNNNNNG11.25No Hit
GTNTAGGAAACCGGCTCGATGAGAGTCCCTTCGTGTCCGGCCTTTCTCTTA11.25No Hit
CGAGCTATTGACTCTACTGTATACATTTTTACTTTTTATGTCCCTCATCGT11.25No Hit
TCGAGAAGGTTCGAAACATCTTGCCGCCAAAAAAACGTACGAAGCGACCTT11.25No Hit
ACTTACGCCAATGTCGTTATCCAGCGGTTCACGGTTGAAACTGTCTCTTAT11.25No Hit
NNNTNNNNNNNNNNNNNNNNNNNNTNNNNNNTNNNNNNNNNNGANNNNNNA11.25No Hit
GANATAGAAATAGTCAAATTTTAAATAGTTTTGTTTTCTTTTTTTTCTTCA11.25No Hit
NNNGNNNNNNNNNNNNNNNNNNNNANNNNNNTNNNNNNNNNNAANNNNNNC11.25No Hit
GGCATCGTTGTTCTTATGGCATTTTAAGTAAGTTATATTCTGGTTGTTCTT11.25No Hit
TGGATATCCACACACTGCAATAGTTTCAGCTACTCAACTGTGGGCATGGCA11.25No Hit
NGGNNNNNNNCNNNNTNNTTGNCNNNNNNANNNNTAGCNNTANTNTTGNTT11.25No Hit
ATACAGAATCATCCACCCATGAGAAAGTGAGAGAGCTGTCTCTTAAACACA11.25No Hit
GACCANNTTCATGGCTAAAACAGTCAGCAAACGGCGTAATCGCCATAGGAG11.25No Hit
GAATTGTTTTANCTAATTGTAGTTTAGATCTTGTTCTTCATGATACAGACT11.25No Hit
TCTCCGTCTTTCTCCATTGCCTCGTGGCCTTGCTATTGACTCTACTGGAGA11.25No Hit
NCTGCTNNGTTNANTTAGGATGTGGCAATNNTNAGCGAACGCTGGTTGAAA11.25No Hit
TTTCTTAACGTATTTAGCCACATAGAAACCAACAGCCATATAACTGGTAGC11.25No Hit
NNNNCNNNCNNNCANNANNCNNNNGANNNANATCACNAAAANNGTCANGCA11.25No Hit
AGTTCCGCCTCATTCCGATGCTGAACAGCTTCTTGGGTAGTAGCAACAGCT11.25No Hit
CTTGTGTTGCACCCGCACCTCGCCAAACGACTCGCCCCAGACCATACCGTT11.25No Hit
NNNCNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNTANNNNNNA11.25No Hit
GTCGTCAACAACTGCCAATTTAATGTAAATACGTACAATGAAAATGTAATT11.25No Hit
AATCTCACATTTTCAACTTTAGCAAAAAAATTGATTTTTTTGAGTTTTGGT11.25No Hit
CCTCTATCCCCATATTGTAAAATATATGCTAACTACTTCAGATGAACCGTA11.25No Hit
GTTTCGGGATGTTCATANCGAAAGCGCCTACTCTTTCCACCCGTTGTGATA11.25No Hit
CTCCCATTCGGATCAAAGAACGTAGTATTAAAATTTCGATCAAATAATAAT11.25No Hit
NNNNNNNNNGANANNNNNNNNACGNNNNNNNNNNNNTNNCGCAANNTNGNT11.25No Hit
NNNANNNNNNNNNNNNNNNNNNNNANNNNNNGNNNNNNNNNNGTNNNNNNA11.25No Hit
GTTGATTATACTAACAAACTTGAAATTACTAAAGTAATTAAAATTCTGTAT11.25No Hit
NNNNNNNNNNNNNGNTNNNNGANNNNNACNTCTNNNNTNNCTNNACGCCTC11.25No Hit
ATTATGCGTCAAATTTTTACTCCAGCTTAATTCGCTCGTTAGTATTTTACC11.25No Hit
NNNTNNNNNNNNNNNNNNNNNNNNANNNNNNTNNNNNNNNNNTGNNNNNNC11.25No Hit
CTCAGCACTAACCTTGCGAGTCATTTCTTTGATTTGGTCATTGGTAAAATA11.25No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATGC50.045.06
GCCTCAA50.045.011
AGCTTCG50.045.037
TTCGTTC50.045.040
AAATCGT50.045.017
TAAGCTT50.045.035
GTTCTTA50.045.043
TTTTTTA50.045.030
TGTTTTC50.045.02
TTTCATG50.045.05
TTTTCAT50.045.04
TTTTTAA50.045.031
GTTTTCA50.045.03
TCGTGGA50.045.020
TGGAGGC50.045.023
TTTTAAG50.045.032
CGTGGAG50.045.021
TGCCTCA50.045.010
TCAAAAT50.045.014
ATGCCTC50.045.09