FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n02_ef1_alpha_2_june.352000000598da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n02_ef1_alpha_2_june.352000000598da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5544324
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG8300.011.7001991
GAGTAGG4503.6379788E-1211.0533581
CCGTCCG2301.1095704E-410.3974659
CGACTAA9950.010.33476610
CTACTCT12450.010.2059514
CATCACG7800.010.0353522
GGACGAC7100.09.9896427
CTAATGC14100.09.8895733
CTGCCGA5200.09.87458624
CCTACAC11650.09.8694693
TATACTG17550.09.7892615
GTATAGC9700.09.7673281
TAGACCG7750.09.7174535
TCTATAC18750.09.5245283
TCACGTC8900.09.5179094
GCCTAAT11000.09.4743081
TACACTG16000.09.4137825
GTGTAGT10600.09.3849271
CTGGACG6300.09.3390695
TAGTACC6300.09.3374554
Produced by FastQC (version 0.11.2)