FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n01_mesp_mekmut_10hpf_3_may.35100000059912.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n01_mesp_mekmut_10hpf_3_may.35100000059912.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26743
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC600.006296769718.7766345
TCCTGTC650.00998988517.33227242
TTATACA952.6627863E-416.60249345
CTTATAC1104.3172284E-516.38687744
TGTCTCT3550.014.59816745
TCTGTCT1150.001136399813.71510243
CTGTCTC4100.013.18943744
TCTTATA1250.002130298412.61789543
CCTGTCT2055.7893783E-510.99119843
GTCTCTT2350.00249823498.6292645
Produced by FastQC (version 0.11.2)