FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n01_mesp_lacz_10hpf_2_june.3510000005993c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n01_mesp_lacz_10hpf_2_june.3510000005993c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58968874
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT86550.023.37427529
CTCCGAG80150.023.35584618
TCCGAGC87550.022.25360319
CCGAGCC91150.020.91821720
GAGACTG95250.020.4614130
CCCACGA99700.020.3636925
CCACGAG98000.020.08115826
GCCCACG96200.020.07734324
AGCCCAC99700.019.84557223
AGACTGC103700.019.1441831
GGGTATC81050.018.9439639
CGAGCCC102650.018.74989521
ACGAGAC111600.018.0672428
CACGAGA109750.017.98303227
CTGGGTA106150.017.6185937
GAGCCCA110200.017.30247522
TGCTGGG108050.017.1009935
ATCTCCG123800.016.26968416
TATCTCG106300.015.61537242
GACTGCT130900.015.30348632
Produced by FastQC (version 0.11.2)