FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n01_gfp_18hpf_20_05_15.351000000598f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n01_gfp_18hpf_20_05_15.351000000598f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17898000
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC46500.016.75049419
CTCCGAG47250.016.34209418
CCGAGCC48300.015.90321520
CAAGAGG48500.015.7848434
AAGAGGC50800.015.11436735
CGAGCCC51600.015.01681421
CGAGACA55700.014.75418929
GAGGCAA53050.014.51568237
GCCCACG53550.014.38625924
AGCCCAC55500.014.36642223
AATCTCG50300.014.35333542
CCACGAG56400.014.34033326
CCCACGA57650.014.29815525
GAGCCCA54100.014.19844722
ACGAGAC59500.013.92511728
CACGAGA59300.013.74485527
CTCGTAT60550.013.70662245
AGAGGCA56100.013.64643136
TCTCCGA62900.013.02546917
ATCTCCG62850.012.99172916
Produced by FastQC (version 0.11.2)