FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n01_gfp_15hpf_23_04_15.35100000059962.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n01_gfp_15hpf_23_04_15.35100000059962.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28349385
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC43200.013.48542245
TATGCCG46700.012.13762443
GTATGCC47950.011.68066842
ATGCCGT49050.011.46437244
AGTCTGA55800.010.34168533
CAGGTAC52850.010.2143143
CGTATGC58250.09.96288941
GTACTAC68150.09.9265073
CTAGTCA58450.09.9085389
GTCCAAG52750.09.89297841
GGTACTG59700.09.7010745
GTATAAG162800.09.6716321
AACCGCG52050.09.4017827
ACCGCGG43800.09.1226358
CTCCGAG69600.09.03073718
AGGTACT59150.08.9723674
TCCGAGC71050.08.878077519
CCAGGTA60150.08.8232012
GTACTGC71200.08.7962716
ACTAGTC66900.08.6570128
Produced by FastQC (version 0.11.2)