FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n01_gfp_10hpf_2_june.35100000059956.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n01_gfp_10hpf_2_june.35100000059956.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATGTTCAGGGCGGTGTGCCAGAACGGTCTGGTCTGCGGCGAGTCGTTT33.75No Hit
GCCGGACACGAAGTGACTCTCATCGATCCTGTTTCCTACACCTGTCTCTTA22.5No Hit
TGGCAGCAACGGAAACCATAACGAGCATCATCTTGATTAAGCTCATTAGGG11.25No Hit
AGCAATCTTGAACACTCATCCTTAATACCTTTCTTTTTGGGGTAATTATAC11.25No Hit
GATCAGAGTAATGTTAAATTTTTTAAAAATTAGTATTGTTTTTAGATTTAT11.25No Hit
GTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGT11.25No Hit
TGGTAACAAAGCACGTAAATATTAAAAGGGAAATACGAGAGAAGCTGAAAA11.25No Hit
CTCTAGATTACCAGTTATTCCTTCTGGAATAACTACCTTGTTACGCCTTTA11.25No Hit
GAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACCTACCG11.25No Hit
CGGATAATCCTCGCGCCGAAAACCGTTCCAAGTCCATCACAAAGCCACCTG11.25No Hit
ACCTTCAATTATGATGACTTCGCAAAGTTTTGTTCACCGTCCATTTTTAAA11.25No Hit
CTATATTATTATTTGATCGAAATTTTAATACTACGTTCTTTGATCCGAATG11.25No Hit
AAGATTGGGAGGCATGAAAACATACAATTGGGAGGGTGTCAATCCTGACGG11.25No Hit
TACTGATAGCATTCTGCGTGTGAATCATTAGCCTTGCGACCCTCGGCAGCA11.25No Hit
CTGACTTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATATACC11.25No Hit
AATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCA11.25No Hit
ACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGG11.25No Hit
GATGTGGATTGGCGATACAAAACAACTTCTGACGCCGCTGCACGATCAGTT11.25No Hit
ATTCTGAATTACCATTATATCTGTAGGAGGAGTCTAATTAGTTCGTTTATC11.25No Hit
CTGGTAGCTATCTGGTTTAGTTCTGTCGGGTATGAATGTCCTTCCACTCCA11.25No Hit
ATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTC11.25No Hit
ACCATGGATGGAAAAAATATAAAGAGGATTTAGCTATTATTCTAATTAAGA11.25No Hit
GGAGGTGGCTTTTTGACCGCCTCCAAACAATTTAGACATGGCGCAACCAGC11.25No Hit
CCGCTAAGATGATCCGGAAAAAATGGAACTTGGATACTTTCGAAGATGGGT11.25No Hit
GGTTATCGCTGGAGACTCCTTCGAGATGAACGCCGTTCGCACTCGCCGTCT11.25No Hit
GTCACTTGCCACTCCTGCGTCGCCCGCATTGCCGGTTCGCGTAGTGCGTCT11.25No Hit
CATCTAGACATACCCTTTTTGTAACTCGCTTCTAAGCCACAAATTCTCGCT11.25No Hit
GCCGGACACGAATTTACTCTCATCGCAGCTGTTTCCTACACTTGACTATTA11.25No Hit
GATAAGCAGTAAAAACAGACGAATGCTCATAACGATACCACTGACCCTCAG11.25No Hit
CCATTTATTTTAGTAAACTCAGAATACAGAATTTTAATTACTTTAGTAATT11.25No Hit
GTCTGGCATGTAACACATATGTGTCGAGGAAGCAATAACGAATGTGGGGGG11.25No Hit
AATACCTGTCTTTTTGGAGTAATTATACTCATCGCGGATATCCTTAAGAAG11.25No Hit
AAGTACGCGTTCTTGCAAATCACCAGAAGGCGGTTCCTGAATGAATGGGAA11.25No Hit
AATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTC11.25No Hit
GCCTCATCAGGGTTAGGAACATTGGAGCCTTGAATGGCAGATTTAATACCA11.25No Hit
AATAAACACTTCCTTCTAATAAACATAAGAACACGCATGTGTTGTAATAAC11.25No Hit
ATTTAACTGTTCTTGTCTATTTAACTTAACTGAAAAGGATCTCTCATTACT11.25No Hit
GGCCTATGGTAACCGGATTTCTTGGGAACCAGATTTCCGCGACACCGTAAT11.25No Hit
CGGTACGTCTACAAGGCGATCAACGGGGCGGTTAAACATTGTCTCAAATGC11.25No Hit
ATCAATGGAAAAATCCAGAAAAAATAGCAAATACGGCTCTTATTGATAATA11.25No Hit
GTTTACGAAAGGTCGCTTCGTCCGTTTTTTTGGCGGCAAGATGTTTCGAAC11.25No Hit
AAGTTATCATAATTGAAGGTTTATTTAGCTCTCCTATTGTAGGGAAAGATC11.25No Hit
CTCTGCAAACGGCTCTTTTCAGAGCCACCCAATTCCTGTCTCTTATACACA11.25No Hit
ATTTGCACGAACGCGTACTTATTCGCCCCCATAATTATGACCAGTGTTTCC11.25No Hit
GTTCACCCGTTCACCGCTGGATAACGACATTGGCGTAAGTCTGTCTCTTAT11.25No Hit
TCCATTAACTTCTCAGTAACAGATACAAACTCATCACGAACGTCAGAAGCA11.25No Hit
CTAATGGCCGTCTTCATTTCCATGCGGTGCACTTTATGCGGACACTTCCTA11.25No Hit
TAACGTATTTAGACACATAGAAACCAACTGCCATATAACTGGTAGCGTTAC11.25No Hit
AGTGTGATCATCTGGTCTCTTGGGAATGAATCAGTCCACGGCGCTAATCAC11.25No Hit
GTCATTTAGCCCCCCCAGCAAACAACTTAACAAATTAATGCCCCCATAGCT11.25No Hit
GTATACATCATTTGCTATACCGAATTCAGAGATTTTGCATTTTAAGTTCTG11.25No Hit
AATCTAGGTAATCTTGCTTCTCTACGTCCAAACCATCAATTAATAAGTAAA11.25No Hit
GTTTATGTTGAAAAGTGAATTAAGTTCATGAAGGATGGTGTTAATGCCACT11.25No Hit
TTCCAAGCAACAACAGGTTTCCGATATTATGCGCCACAGGCTTACTCAAGC11.25No Hit
GGGATGCACCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACC11.25No Hit
GTCACTTGCCACTCCTGCGTCGCCCGCCTTGCCGGTTCGCGTAGTGCGTCT11.25No Hit
AGACAATACAGATGATTTTAATTGGTCTCTAAAAAAAGGAGCAACTTCTTC11.25No Hit
GTTGTAGATAGAGTAAAATTAATTTTAAAAAATTTATTAGTTTTAGTTAAT11.25No Hit
TCCCATGCCCACAGTTGAGTAGCTGAAGCTATTGCAGTGTGTGGATAAGCA11.25No Hit
TGCCGCAAGCTTGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTAT11.25No Hit
TCATGATTGAATCGCGAATGGTCGGCATATTGCGATCAACGTTCACATTAA11.25No Hit
ACACCCAGTTTATCAAACCCAGGACTAATAGTTTTAGACAGCACGTAAGGG11.25No Hit
ACAAGCCGCAATCGCAGATTTAAGAGCCTCGATACGCTCAAAGTCAAAATA11.25No Hit
GTCGTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGC11.25No Hit
AGGGACGTCCTCATCAGGGTTAGTAACATTAGAGCCTTGAATGGCAGATTT11.25No Hit
GTGTTTATACCAAAACTCAAAAAAATCAATTTTTTTGCTAAAGTTGAAAAT11.25No Hit
CTCCACTCTGAACTCGCTTAGAGGCTTTGCTATTCAGCTTTGGATGAATAC11.25No Hit
GCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCT11.25No Hit
ATTCTCTAAATGTTCTTTGTTTTTTACTAAATATGACGAGAAAGTAGAACG11.25No Hit
GTTTTTCTCTTGTGTTAACGTGGATAGAAAACAAAGAATTCATTTTAACTC11.25No Hit
GGTGTGGACACTAATGGACAATCAGCTAAAACAAACATCATTGTTATTTGT11.25No Hit
CGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAATCGAAATCATCT11.25No Hit
CCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATA11.25No Hit
ATGTGTTACAATTAAATATTTTTAATTTAACTTACTATTTTAGAATGTTAT11.25No Hit
CTCTCTCACTTTCTCATGTGGGTATGATTCTGTATCTGTCTCTTATACACA11.25No Hit
GGACAGGATGCCTCTAATGATAACTCGCTTCATGTGAATGAATAAGAACAA11.25No Hit
GTCACCAGCTGAGTGAGTAATAGCTACCGTGCAAAATAAAACAAGGGCTAA11.25No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA50.045.041
ACAAACT50.045.025
AACGTCA50.045.039
AAACTCA50.045.027
ACAGATA50.045.019
AGAAGCA50.045.045
GATACAA50.045.022
ACGTCAG50.045.040
CAGTAAC50.045.014
GAACGTC50.045.038
CACGAAC50.045.035
CATCACG50.045.032
ACTTCTC50.045.08
ACTCATC50.045.029
AACTTCT50.045.07
AACTCAT50.045.028
TCAGTAA50.045.013
TAACTTC50.045.06
CATTAAC50.045.03
TCACGAA50.045.034