FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l02n01_ef1_alpha_2_june.351000000598dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l02n01_ef1_alpha_2_june.351000000598dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5544324
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG9800.012.8305087
ACCGCGG8550.012.6054128
GACGGAC5650.012.32589432
GGACTCC5150.011.77454735
GCGGTAG8450.011.45252911
CCGAGCC6350.011.320320
AGTCTGA12050.011.18283433
ACGGACT6300.011.05118433
CTGGACG8350.011.0250175
CGGACTC6550.010.97226734
GACCATA12850.010.65928840
CCCACGA8250.010.61969225
GAGTAGG7100.010.5964351
ATCTCCG12750.010.5872145
CCGCGGT9800.010.3102299
CGGTAGC8950.010.30980612
GGACGAC9600.010.2911377
CGCGGTA9950.010.1783910
TGGACGA10450.010.0986536
CTATACT20400.010.054894
Produced by FastQC (version 0.11.2)