FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n02_mesp_mekmut_10hpf_14_june.35200000059946.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n02_mesp_mekmut_10hpf_14_june.35200000059946.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22131540
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC916550.4141374707769997Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC127150.034.44148336
AGATCTC133200.032.70863338
TCTCGGT134900.032.1983241
TCGGTGG143200.030.37805243
CGAGTGT141350.030.30974431
GACGAGT143500.030.05872529
CTGCCGA146400.029.89066924
GAGTGTA149700.029.49212332
TAGATCT149400.029.47763637
ACGAGTG145950.029.47739430
GTGTAGA151200.029.08074634
CTCGGTG149150.029.0164242
GGTGGTC152550.028.6194745
ACGCTGC156450.028.53166621
CGCTGCC156650.028.48081222
ATCTCGG155550.028.06877340
CGGTGGT156450.027.9347544
GCTGCCG159950.027.76661923
TGTAGAT158550.027.64741335
AGTGTAG159300.027.60100233
Produced by FastQC (version 0.11.2)