FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n02_mesp_lacz_10hpf_21_april.35200000059902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n02_mesp_lacz_10hpf_21_april.35200000059902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43983383
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC1493070.339462291929659Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT205650.034.8770541
TCGGTGG208200.034.4811343
CTCGGTG209350.034.2283942
CTGCCGA217150.033.22812324
GACGAGT216800.032.74476629
CCGACGA217650.032.61666527
GCTGCCG224250.032.226423
ATCTCGG224050.031.9829440
CGCTGCC228400.031.78846522
CGGTGGT230350.031.41950644
ACGAGTG229000.031.10900730
ACGCTGC240300.030.27979521
GTAGATC241450.030.19732936
CGAGTGT237500.030.16661531
GCCGACG235300.030.1223926
TGCCGAC235150.030.07222725
AGATCTC241550.029.90612438
CTGACGC249400.029.58337818
GTGTAGA248000.029.42589834
TGACGCT251650.029.25628519
Produced by FastQC (version 0.11.2)