FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n02_mesp_dnfgfr_10hpf_26_april.35200000059989.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n02_mesp_dnfgfr_10hpf_26_april.35200000059989.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19597629
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC849050.43324118443103504Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG115600.036.2685943
TCTCGGT119950.035.1043841
CTCGGTG120600.034.7845542
CCGACGA123900.033.63332727
CTGCCGA129050.033.03824624
GACGAGT126400.032.98599629
GCTGCCG130600.032.8531223
ATCTCGG129300.032.70539540
CGGTGGT131950.032.1326944
CGCTGCC133850.031.97136722
AGATCTC132750.031.88721338
GCCGACG131250.031.78431126
CGAGTGT131900.031.73084331
GTAGATC135800.031.48525636
TGCCGAC133950.031.19148425
ACGCTGC138250.031.10030621
ACGAGTG134500.031.08336430
GATCTCG140100.030.35974939
GTGTAGA140800.030.25405734
GGTGGTC139700.030.15670845
Produced by FastQC (version 0.11.2)