FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n02_gfp_10hpf_28_april.3520000005992c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n02_gfp_10hpf_28_april.3520000005992c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52463865
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC896860.17094813735129885Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGT146950.028.81705529
TCTCGGT148200.028.70145841
CCGACGA154500.027.42324827
CTCGGTG158950.026.57627342
GTAGATC164300.026.18786236
ATCTCGG165800.025.6277940
AGATCTC167550.025.51913538
CGCTGCC178650.024.20569822
GAGTGTA185850.023.47704732
CTGCCGA183150.023.37757924
ACGAGTG185950.023.2091230
GCTGCCG189450.023.00402823
TCGGTGG189900.022.68266543
GACGCTG197200.021.89451420
GATCTCG207900.021.02185239
TGCCGAC219900.019.98237625
ACGCTGC217300.019.7968221
CGACGAG221300.019.38958228
GCCGACG221100.019.2849826
GGTGGTC234200.018.3728345
Produced by FastQC (version 0.11.2)