FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n02_ef1_alpha_16_june.352000000598e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n02_ef1_alpha_16_june.352000000598e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29944825
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC421920.14089913699612538Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT83950.024.8516541
AGATCTC85950.024.27093738
CTCGGTG86850.023.84043742
TCGGTGG88450.023.61210643
GTAGATC90550.023.55933836
CTGCCGA90500.023.22012324
GCTGCCG92300.022.74306923
CGCTGCC95600.021.91086222
CCGACGA94300.021.90436427
GACGAGT98300.021.24200629
ATCTCGG100150.020.87676440
GATCTCG102950.020.3079839
ACGAGTG104500.020.08957730
GGTGGTC107000.019.79200245
CGGTGGT110150.019.10344544
GCCGACG109300.019.02185626
CTGACGC112100.018.98838618
CGAGTGT110400.018.91438931
TGCCGAC113600.018.75596225
ACGCTGC114850.018.59100521
Produced by FastQC (version 0.11.2)