FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n01_mesp_mekmut_10hpf_14_june.35100000059949.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n01_mesp_mekmut_10hpf_14_june.35100000059949.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22131540
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTAT935780.42282642780393953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCCG126900.034.9119231
GACCCGT129650.034.3103232
TATCTCG127150.033.2852142
ACCCGTT135250.032.9229833
CTCGTAT137350.032.1727945
TCCGAGC143400.031.34790419
GAGACCC143250.031.11534130
ACGAGAC144000.031.01564428
GTATCTC137000.030.99117541
GCCCACG143750.030.975624
CCCACGA144250.030.7435225
CCGAGCC146500.030.56177720
CGAGCCC146550.030.45930321
CCGTTTG147750.030.32036235
TCTCGTA141000.030.17507444
AGCCCAC148150.030.1467623
CCACGAG147750.030.12184526
CACGAGA149900.029.8849427
CTCCGAG152650.029.47775718
CCCGTTT154000.029.27976634
Produced by FastQC (version 0.11.2)