FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n01_mesp_lacz_10hpf_21_april.35100000059905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n01_mesp_lacz_10hpf_21_april.35100000059905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43983383
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTAT1530170.3478972956673205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTA218550.033.87187232
GCTACGC221400.033.30377635
TACGCTA221550.033.04767637
TCCGAGC226750.033.02137419
CGAGACG226050.032.91663429
CCACGAG225750.032.84036626
CCGAGCC227600.032.7894620
CCCACGA227050.032.7018825
CGCTACG226000.032.69560234
GCCCACG226850.032.67116524
CTACGCT226200.032.44785736
CTCCGAG230700.032.348618
AGCCCAC230400.032.2262923
TATCTCG219450.032.0303142
CGAGCCC233900.031.8004821
AGACGCT234150.031.7592331
ACGCTAC236250.031.47706833
CGCTATC223250.031.4652839
GAGCCCA241000.030.90152522
CTATCTC228150.030.73997741
Produced by FastQC (version 0.11.2)