FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n01_mesp_dnfgfr_10hpf_26_april.3510000005998c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n01_mesp_dnfgfr_10hpf_26_april.3510000005998c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19597629
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT827770.42238272803306975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC127100.033.86356720
CCACGAG128050.033.50827826
TCCGAGC128900.033.40788719
GCCCACG129050.033.1963224
CAAGAGG128650.033.17778834
AGCCCAC131350.032.7177323
CTCCGAG131400.032.63529218
CCCACGA132350.032.5726125
AAGAGGC131250.032.5719835
CGAGACA132400.032.4754229
CACGAGA133050.032.28286427
ACGAGAC133500.032.2246928
GAGGCAA132950.032.17249737
AGAGGCA134300.031.76524436
CGAGCCC135950.031.67568621
GAGCCCA137750.031.42534822
AATCTCG129750.031.26586742
GCAATCT132100.030.69286340
CTCGTAT146500.029.1039245
GAGACAA148150.028.99269930
Produced by FastQC (version 0.11.2)