FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n01_gfp_10hpf_28_april.3510000005992f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n01_gfp_10hpf_28_april.3510000005992f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52463865
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT859070.16374508435472682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA130000.032.2445330
ACTAAGG133800.031.27864533
CGAGACT136300.030.83647229
GACTAAG142950.029.4811732
GCGAATC138400.028.93824239
AGCCCAC151150.028.23839223
CGAGCCC152050.028.0998221
CCACGAG154600.027.4045126
CTCCGAG156450.027.22294618
GAGCCCA157950.027.10771222
CACGAGA158200.026.9516127
CCCACGA159350.026.68645725
CTAAGGC160900.026.02444334
AATCTCG156850.025.90681542
CGAATCT164700.024.39913440
TCCGAGC184500.023.95007119
CCGAGCC186050.023.61767820
GAATCTC172150.023.4477741
CTCGTAT186750.022.56604645
ACGAGAC188950.022.52978728
Produced by FastQC (version 0.11.2)