FastQCFastQC Report
Thu 23 Jun 2016
HJ5TMBCXX_l01n01_ef1_alpha_16_june.351000000598ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TMBCXX_l01n01_ef1_alpha_16_june.351000000598ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29944825
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT399100.1332784546244635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTC86450.024.17901630
AGACTCC86500.024.11333831
TCCGAGC95300.022.94718219
CCTGAGC92950.022.39172436
CGAGACT93600.022.33193229
CCGAGCC97200.022.31356220
GCCCACG97050.022.23347524
AGCCCAC99600.021.8675623
GACTCCT96500.021.63793832
CCACGAG100050.021.45439526
CCCACGA100100.021.39868725
CTCCGAG101250.021.30980918
CGAGCCC104000.020.9195721
TCCTGAG102900.020.68573235
GAGCCCA106000.020.58908822
CATCTCG101350.019.98034742
CTCCTGA106200.019.55565534
ACGAGAC113900.019.28024528
GAGCATC104050.019.24589239
CACGAGA113450.019.07903127
Produced by FastQC (version 0.11.2)