FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27020144
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA579030.21429567510817116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG43250.029.3063051
TCCCTAC26200.014.7900119
CTAGTTG23400.014.7694588
CCCTACA36150.014.00284110
GCACACG90850.013.8332038
ACGTCTG92150.013.71582412
TCGGAAG74650.013.7113843
CACACGT93350.013.5749789
GTACTCT24450.013.5733863
CCTACAC35000.013.06806111
GTTACCT23850.013.02914614
ACACGTC97100.013.01504810
TACACGA27650.012.88007913
AGCACAC97150.012.8283447
ACCTACT16400.012.81083533
CGGAAGA103100.012.769321
CAGTCAC99650.012.53457524
AGAGCAC105050.012.4035665
TACTCTA28050.012.2047884
CTAATGC26350.012.19675321
Produced by FastQC (version 0.11.5)