FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_9_nlp7_stomata_N_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_9_nlp7_stomata_N_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16548085
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT248460.1501442614054738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA27850.019.97206510
GCCGGGT25600.018.5884511
CGGGGGG7150.018.1067261
CTACACG27800.017.61709612
TCCCTAC31250.017.4632729
CGGGGGT7500.016.7952121
CGGGCAT14200.016.262971
TACACGA31450.015.23899213
CCTACAC35950.014.79098311
TAGGGTA63250.014.38442531
CGGGCGT13450.014.30818751
GGGGCAT17500.013.7960661
CGTAATA135650.013.33341815
CGCGGAT88350.013.3069021
TTCCCTA48700.012.7142328
GTAATAG143050.012.69243516
GGGGAAT82450.012.3918581
TAATAGT147550.012.35335717
CGGGGCC6000.012.2465091
TATTTCG68850.012.1978830
Produced by FastQC (version 0.11.5)