FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_8_nlp7_stomata_N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_8_nlp7_stomata_N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15327533
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT208650.13612758165322494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG11000.020.0399721
CCCTACA30650.018.489610
GCCGGGT31250.016.9073731
TCCCTAC32550.016.6580319
CTACACG28850.016.1269812
CCTACAC39550.014.50580111
TACACGA32350.014.38236213
CGGGCAT16950.014.243891
CGTAATA108050.014.18131215
GCGGGGG14050.013.9463621
TAGGGTA48600.013.39183531
GTAATAG116650.013.28579216
GTCCGCT12250.013.1392621
CGCGGAT72400.013.0489161
GGGGAAT79900.012.8750761
TAATAGT121500.012.69828217
CGCCCAA77200.012.518476558
TATTTCG61500.012.40359630
CCTGTAA63100.012.25944838
AAGTGCG72450.012.22697855
Produced by FastQC (version 0.11.5)