FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_7_nlp7_stomata_N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_7_nlp7_stomata_N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837047
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTATTGAGTGACGTAATAGTTGAGACCAAGAAAATCATATGAACCCT119040.1347056318700127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG5750.018.8668861
CGCGGGG4550.017.6897961
CCCTACA14700.016.1831410
CGCGGAT46550.014.8099281
CGCGTAT6050.014.4607571
TACACGA15500.014.44514713
GCCGGGT22250.014.3125761
GGGGTAT7850.014.2654911
GTCCGCT7950.014.0860511
CGTAATA67650.013.70437315
TCCCTAC16450.013.6108529
GGGGAAT50250.013.4408791
GCGGATC54650.013.381312
TAGGGTA34600.013.35017731
CGGGGAG13050.013.1398981
GTAATAG71850.013.09812316
CTACACG14900.012.91353712
TAATAGT73050.012.78768117
AGGCGAG32650.012.54665356
GGGGCAT10900.012.5211581
Produced by FastQC (version 0.11.5)