FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_6_wt_stomata_control_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_6_wt_stomata_control_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9957545
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA24450.017.31255710
CTACACG21150.016.8710612
CGGGGGG6700.016.191281
TCCCTAC24600.016.069349
CCTACAC30000.014.6927811
TACACGA27250.013.736313
GCGGGAC8450.012.8380571
ACACGAC28000.012.36892214
CGCGGGG9400.012.2851481
GGGGCAT13800.011.6646861
GCGGGAT31200.011.3282051
CGGGGAT25050.011.3154441
GCCGGAT28500.011.05075551
CGGGGGT8300.010.9619931
TTCCCTA41900.010.2693158
ATCGGGC16100.010.2131956
CCGGGGT8400.09.9983021
CGGGAAT30400.09.8996351
CCGGCAT22400.09.8420791
CGCGGAT24600.09.8154061
Produced by FastQC (version 0.11.5)