FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_5_wt_stomata_control_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_5_wt_stomata_control_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10822615
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT6800.014.921781
CTACACG21750.013.83140812
TCCCTAC23350.013.7825679
CCCTACA27200.013.11778410
CCTACAC27800.013.08626611
CCGGGAT31700.012.2516681
CGGGGGG8000.012.2461491
GCCGGGT21450.012.0707971
GCGGAAT21900.011.9825341
CGGGCAT13900.011.5790941
CCGGGGT10200.011.319971
GGGGCAT12700.011.0201581
GCCGGAT28200.010.7944781
CGGGGGA13500.010.6262881
CTAGTTG31350.010.6001318
ACACGAC29450.010.5715314
CTGTAGG19850.010.40744949
GCGGGCT20100.010.0963281
CCCGGAT20150.010.0712751
CACCTAC20750.09.95088732
Produced by FastQC (version 0.11.5)