FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_4_wt_stomata_control_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_4_wt_stomata_control_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16999016
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG12850.020.1499181
CGGGGGT10450.015.067491
CCCTACA37100.014.23822110
GCCGGGT31450.013.016971
CCTACAC44400.012.9214811
GCGGGGG15850.012.8039331
TCCCTAC41150.012.6668639
CCGGGGG12200.012.0457581
CTACACG35800.011.72609712
CCGGGAT49400.011.4744741
GGGGAAT57550.011.247891
GCGGAAT31200.010.878321
CGGGCAT19800.010.7797661
GCGGGAT35450.010.6598771
CGCGGAT37750.010.6592241
CGGGTAT28450.010.576961
GCGGGGT15100.010.4275021
GCGGGAG35000.010.3970461
TACACGA43550.010.36240713
CCCGGAT31200.010.3175811
Produced by FastQC (version 0.11.5)