FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_3_wt_stomata_N_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_3_wt_stomata_N_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12445639
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA25550.017.6623810
TCCCTAC27700.015.6600229
GCACACG21050.015.45543711
CGGGGGG10300.015.2879011
CCTACAC31800.014.19100111
CTACACG27100.013.29597312
GCGGGGT11250.013.0637951
CGCGGGG8450.012.8374151
GCGGGGG10700.012.7542061
GCCGGGT20350.012.0366671
TATGCCG21450.011.75284948
CGGGGAT31300.011.7386341
ACGTCTG25650.011.7295815
CGCGGAT28950.011.6036671
CCCCCAT11750.011.3166611
CACACGT26950.011.29308712
TACACGA33500.011.17368413
GGGGAAT41100.011.1532661
CCGGGAT35550.011.1227311
ACACGAC36000.010.883697514
Produced by FastQC (version 0.11.5)