FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_2_wt_stomata_N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_2_wt_stomata_N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18841899
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG15050.019.761921
CCCTACA38950.016.07656310
TCCCTAC41250.013.8232859
CCTACAC48100.013.23645311
GCGGGAT37700.012.9009051
CGGGGGT13350.012.5807551
CTACACG40650.012.56424612
GCGGGGG18900.012.4039421
CGCGGAT44900.012.2348881
GGGGAAT62550.012.0270161
CGGGCAC18800.011.539331
CCGGGAT53400.011.270261
TACACGA50250.011.20826913
GCGGAAT36250.011.0038221
GCCGGGT32150.010.9922631
CGCGGGG14500.010.85903551
CGGGGAT47150.010.8347251
GCGGGGT20700.010.81823351
CGGGCAT23350.010.6394251
GCCGGAT51400.010.2792291
Produced by FastQC (version 0.11.5)