FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_24_nlp7_no_stomata_control_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_24_nlp7_no_stomata_control_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12896704
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC315450.24459737929939307TruSeq Adapter, Index 9 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194400.1507361880989127No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT166290.12893992139386934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG61050.038.62319615
CGTATGC58100.037.8399644
CTATAAT59700.037.16583633
TATGCCG59800.036.6494346
CACACGT65800.035.78049512
GTATGCC62100.035.51424445
GCACACG68450.035.1617511
CACTATA63050.034.90817631
ACTATAA63950.034.42103632
TATAATA65000.034.35195534
TCGTATG65250.034.06907343
AGCACAC70550.033.76801710
CTCGTAT65500.033.6695842
TATCTCG65450.033.53289439
CACGTCT71300.033.266414
AGTCACT69250.031.78664428
TAATATC72350.030.76851336
ACACGTC76600.030.73620213
CAGTCAC75450.029.35980827
CCAGTCA77000.028.6774626
Produced by FastQC (version 0.11.5)