FastQCFastQC Report
Tue 12 Nov 2019
HJ5TLBGXC_n01_23_nlp7_no_stomata_control_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TLBGXC_n01_23_nlp7_no_stomata_control_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6601367
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96430.1460758052082243No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC82040.12427728984011949TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19300.035.7336644
ACGTCTG23200.034.0789615
GTATGCC20950.032.91682445
CACACGT24950.031.68817312
TATGCCG21750.031.22680546
CTCGTAT22450.029.62554742
GCACACG28500.027.74104711
TCGTATG24050.027.36579543
AGCACAC29250.027.26894210
CACGTCT29700.026.9737714
AGTCACT28000.026.6224928
ACTACAG27750.026.48318732
CACTACA28450.026.32161531
ACACGTC30750.025.82498413
CGTCTGA33800.023.80566216
CAGTCAC31450.023.59024427
GTCACTA31850.023.29185929
TCACTAC31900.023.25605830
CTGAACT35850.022.73766119
CTACAGC34150.022.03339433
Produced by FastQC (version 0.11.5)